1.1 Find a mold folder and take out one working page.Need to determine if there is two finish working color markers. If only one, need to finish it priority.
1.2 Open the data sheet and find the number of the page.
For North quantity test: https://docs.google.com/spreadsheets/d/166n-3RT2ai1lQwvxABFdsAe6WKWF4I5sx1N6Zqs4soU/edit?usp=drive_link
For South quantity test: https://docs.google.com/spreadsheets/d/1xihtO0SSQLMLCAdFZAFwq3XT1wGSoP9B/edit?usp=drive_link&ouid=102207485550371806190&rtpof=true&sd=true
For north garden: https://docs.google.com/spreadsheets/d/1xihtO0SSQLMLCAdFZAFwq3XT1wGSoP9B/edit?usp=sharing&ouid=102207485550371806190&rtpof=true&sd=true
For south garden: https://docs.google.com/spreadsheets/d/1l0IMaZir0SRKR14aO3ckYuN5QF2ONtCX/edit?usp=drive_link&ouid=102207485550371806190&rtpof=true&sd=true
1.3 Take an unlabeled grid sheet and copy the sample IDs and any notes (most sheets will not have notes) from the labeled grid sheet to the unlabeled grid sheet.
1.4 Put the tray on the table, the label should be on the upper left corner.
1.5 Move the samples from the labeled grid sheet to the preparation area in the metal pallet in the same order. Only move one sample at each time, and put them facing up.
1.6 Check that the sample is clean and clean with paper towels if needed. If needed, can use ethanol to clean the surface, but need to use the tissue to dry it before the next step.
1.7 Add the number ID on the data sheet *
YYYY_region_row_col_surface (if doing a single image per
mold) * YYYY_region_row_col_surface_rep (if doing a
multiple images per mold) * Use _, no whitespace * Always
use lowercase * col – always use three digits
Examples:
2.1 Open a bottle of nail polish and make sure the brush is clean. Make sure close it if don’t use it.
2.2 Apply an even coat of nail polish to each mold and put them back to the working place (full cove the mold, and as thin as possible).
Close the nail polish cover when finish used.
2.3 Wait for drying, about 10 minutes.
2.4 Apply the dried nail polish with transparent tape and remove it from the mold (The tape could be a little longer than tape. Fold the bottom end, so it will be easy to remove it later.)
2.5 Apply tape and nail polish to the slide, make it as flat as possible. Use a black marker to carefully record the sample number on the tape. Example:
3.1. Computer and microscope
3.1.1 If the microscope has a cover, remove it carefully.
3.1.2 Open the light of the microscope (check the light cover is full open)
3.1.3 Move the side lever to the middle position